Catalogue Number: AB05126-1.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | LPS; Kdo(2 to 8)Kdo(2 to 4)Kdo |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | S25-23 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised by immunizing a BALB/c mouse with KD03-GlcNAc-BSA. |
| Application: | ELISA, WB, FA |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Multiple different formats of this antibody were used for surface plasmon resonance on alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo, it showed an affinity between 4.5 * 10^4 M^-1 * s^-1 and 5.9 * 10^4 M^-1 * s^-1 (Mueller-Loennies et al., 2000; PMID:10642603). This antibody was used for an ELISA on KDO3-GIcNAc-BSA to characterize this antibody. This antibody was used for a western blot on chlamydia LPS, it showed it was able to bind to r595-207 LPS (Fu et al., 1992; PMID:1372290, Haji-Ghassemi et al., 2018; PMID:30571096, Brade et al., 1997; PMID:9376684). The structure of this antibody was determined using x-ray crystallography (Haji-Ghassemi et al., 2018; PMID:30571096).