Anti-Porphyrin [14H7], Mouse IgG1, lambda

Catalogue Number: AB05130-1.1-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Shipping Condition:Blue Ice
Unit(s): 1 mg
Host name: Mouse
Clone: 14H7
Isotype: IgG1
Immunogen: This antibody was raised by immunizing a BALB/c mouse with Fe(ToCPP)-KLH.
Application: ELISA, FA

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The structure of the Fab fragment of this antibody was determined using x-ray crystallography (Robles et al., 2012; PMID:23240001). This antibody was used for an competitive ELISA on porphyrin, it showed that it bound most strongly to (Ortho-COOMePh)4-porphyrin (De Lauzon et al., 1998; PMID:9799110, Quilez et al., 1996; PMID:8849692). This antibody was used for an peroxidase activity experiment, this antibody showed the ability to catalyse the oxidation of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by H202 (Quilez et al., 1996; PMID:8849692).