Anti-Factor VIII [BO2C11], Mouse IgG1, kappa

Catalogue Number: AB05132-1.1-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	AHF; Coagulation factor VIII; Antihemophilic factor; Procoagulant component
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Mouse
Clone:	BO2C11
Isotype:	IgG1
Immunogen:	This antibody was raised from PMBCs from a hemophilia A patient with inhibitor to fVIII for more than 10 years.
Application:	ELISA, WB, FA, InVitroA, InVivoA, SPR

Overview
Datasheets

Additional Text

Gene Name

Uniprot ID

P00451

Gene ID

2157

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original antibody was used for an ELISA on factor VIII, in a further ELISA it was also shown that the antibody inhibits factor VIII binding to vWF (Jacquemin et al., 1998; PMID:9657749, Gangadharan et al., 2017; PMID:27758819, Villard et al., 2003; PMID:12676786). This antibody was used for a western blot on reduced factor VIII. This antibody was evaluated in an inhibition assay to assess its ability to suppress plasma factor VIII activity. Using a coagulation assay, it was demonstrated that the antibody reduces factor VIII activity by 50% at a concentration of 0.14 g/mL (Jacquemin et al., 1998; PMID:9657749, Villard et al., 2003; PMID:12676786). In a functional assay it was confirmed that vWF can inhibit the factor VIII inactivation by this antibody, but this effect decreases with incubation time. This antibody was used for surface plasmon resonance on factor VIII (Jacquemin et al., 1998; PMID:9657749). This antibody was used for an in vivo experiment, it was shown that this antibody can cause a 70% reduction in factor VIII internalization by MoDCs. This antibody was used in vitro on dendritic and CD4+ T cells, it was shown that is was able to diminish factor VIII immunogenicity (Gangadharan et al., 2017; PMID:27758819). This antibody was used in vivo on factor VIII deficient mice, they were injected with 0.5 IU in 100 µL physiologic saline of human factor VIII, it was shown that 0.25 ug of this antibody was sufficient to inhibit the factor VIII (Villard et al., 2003; PMID:12676786). The structure of the fab version of this antibody was determined using x-ray crystallography (Spiegel Jr et al., 2001; PMID:11418455).

AB05132-1.1-BT Datasheet

Price

£1,825.00

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