Anti-MenX capsular polysaccharide [MenX.01], Mouse IgG1, kappa

Catalogue Number: AB05150-1.1-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Neisseria meningitidis serogroup X capsular polysaccharide; MenX-CPS
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Mouse
Clone:	MenX.01
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BALB/c mice with a glycoconjugate of the Neisseria meningitidis serogroup X polysaccharide with the CRM197 carrier protein (MenX-CRM197).
Application:	ELISA, WB, NT, SPR, NMR, ITC, Crstapy

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original antibody format (mouse IgG1, kappa) was generated, and its specificity was verified using ELISA, demonstrating specific binding to MenX capsular polysaccharide (CPS) with no cross-reactivity to MenA-PS, GBS-PSII, or CRM197 protein alone. It was used in competitive ELISA with oligosaccharide (OS) fragments (DP5.5-40), showing full inhibition of CPS binding at DP5.5, indicating the minimal epitope. The antibody exhibited bactericidal activity in the rabbit serum bactericidal assay (rSBA) with a titer of 1,024 at 0.98 µg/ml against MenX strain Z9516. Surface plasmon resonance (SPR) analysis revealed a submicromolar affinity (Kd ~ 0.32 µM) to MenX-CRM197 conjugate, with fast on/off rates. STD-NMR with a DP7 fragment identified strong interactions involving H-1 and H-4 protons near phosphodiester bridges. Isothermal titration calorimetry (ITC) confirmed entropically driven binding with Kd values of ~2-3 µM for DP5.5-7 and ~0.3 µM for DP15. Western blot/immunoblot confirmed specific detection of MenX-CRM197 conjugate bands. A Fab version of this antibody was generated and tested for functionality and binding in ELISA, confirming specific recognition of MenX CPS. SPR analysis of Fab showed a comparable affinity (Kd ~ 0.54 µM) to the full mAb. An IgG2a recFab-His format of the antibody was constructed and crystallized, yielding a 2.16 Å resolution structure (PDB: 7OO2), which was used for in silico docking with a DP6 OS, revealing hydrogen bonds, salt bridges, and hydrophobic interactions involving all six repeating units (Pietri et al., 2021; PMID: 34722634). The findings on the minimal epitope (5-6 repeating units) were supported by a separate study on synthetic MenX-related phosphooligosaccharides, where trimeric and longer (avDP12) conjugates elicited anti-MenX IgG and bactericidal activity, though weaker than natural polysaccharide conjugates (Khatuntseva et al., 2025; DOI: 10.3390/molecules30153068).

AB05150-1.1 Datasheet

Price

£430.00

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