Anti-MenX capsular polysaccharide [MenX.01], Human IgG1, kappa

Catalogue Number: AB05150-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Neisseria meningitidis serogroup X capsular polysaccharide; MenX-CPS
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Human
Clone:	MenX.01
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BALB/c mice with a glycoconjugate of the Neisseria meningitidis serogroup X polysaccharide with the CRM197 carrier protein (MenX-CRM197).
Application:	ELISA, WB, NT, SPR, NMR, ITC, Crstapy

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original antibody format (mouse IgG1, kappa) was generated, and its specificity was verified using ELISA, demonstrating specific binding to MenX capsular polysaccharide (CPS) with no cross-reactivity to MenA-PS, GBS-PSII, or CRM197 protein alone. It was used in competitive ELISA with oligosaccharide (OS) fragments (DP5.5-40), showing full inhibition of CPS binding at DP5.5, indicating the minimal epitope. The antibody exhibited bactericidal activity in the rabbit serum bactericidal assay (rSBA) with a titer of 1,024 at 0.98 µg/ml against MenX strain Z9516. Surface plasmon resonance (SPR) analysis revealed a submicromolar affinity (Kd ~ 0.32 µM) to MenX-CRM197 conjugate, with fast on/off rates. STD-NMR with a DP7 fragment identified strong interactions involving H-1 and H-4 protons near phosphodiester bridges. Isothermal titration calorimetry (ITC) confirmed entropically driven binding with Kd values of ~2-3 µM for DP5.5-7 and ~0.3 µM for DP15. Western blot/immunoblot confirmed specific detection of MenX-CRM197 conjugate bands. A Fab version of this antibody was generated and tested for functionality and binding in ELISA, confirming specific recognition of MenX CPS. SPR analysis of Fab showed a comparable affinity (Kd ~ 0.54 µM) to the full mAb. An IgG2a recFab-His format of the antibody was constructed and crystallized, yielding a 2.16 Å resolution structure (PDB: 7OO2), which was used for in silico docking with a DP6 OS, revealing hydrogen bonds, salt bridges, and hydrophobic interactions involving all six repeating units (Pietri et al., 2021; PMID: 34722634). The findings on the minimal epitope (5-6 repeating units) were supported by a separate study on synthetic MenX-related phosphooligosaccharides, where trimeric and longer (avDP12) conjugates elicited anti-MenX IgG and bactericidal activity, though weaker than natural polysaccharide conjugates (Khatuntseva et al., 2025; DOI: 10.3390/molecules30153068).

AB05150-10.0 Datasheet

Price

£430.00

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