Anti-Robo4 [71.22.S1.16], Rabbit IgG, kappa

Catalogue Number: AB05153-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Roundabout homolog 4; Magic roundabout; YW71.22.S1.16
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Rabbit
Clone:	71.22.S1.16
Isotype:	IgG
Immunogen:	The original antibody is derived from phage display mutagenesis and selection.
Application:	FACS, FC, SPR

Overview
Datasheets

Additional Text

Gene ID

54538

Gene Name

ROBO4

Uniprot ID

Q8WZ75

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original format of the antibody bound human and murine Robo4 expressed endogenously in human HUVEC cells and murine MS1 cells by FACS analysis. The original antibody inhibited HUVEC tube elongation. Binding affinities of the antibody to human and mouse Robo4 were determined by SPR measurements for the IgG1 format of the antibody (KD= 1.1 nM and 0.9 nM for human and mouse respectively), the IgG1 format of the antibody with engineered Cys (KD= 1.2 nM and 1.1 nM for human and mouse respectively), and a Fab fragment with engineered Cys (KD= 8 nM and 1.5 nM for human and mouse respectively). Binding affinities of the antibody to human and mouse Robo4 were determined by radiolabeled cell binding for the IgG1 format of the antibody with engineered Cys(KD= 1.2 nM and 1 nM for human and mouse respectively), and a Fab fragment with engineered Cys(KD= 3.2 nM for mouse) (US7834154B2).

AB05153-23.0 Datasheet

Price

£430.00

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