Anti-CD172a [035c], Rabbit IgG, kappa

Catalogue Number: AB05165-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	100 ug
Host name:	Rabbit
Clone:	035c
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing Balb/c and SJL/J mice by three different strategies: protein immunization using human SIRPα v1 ECD recombinant protein, cell immunization using 293F cells stably expressing human SIRPα v1 and genetic immunization using gold bullets coated with human SIRPα v1 expression plasmid.
Application:	ELISA, FACS, FC, Blk

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The specificity of the original format of the antibody to human SIRPα v1 ECD was confirmed by ELISA analysis. The original format of the antibody bound to 293F cells stably expressing human SIRPα v1 by FACS assay. The original format of the antibody stimulated potent macrophage phagocytosis of Jurkat cells and Rai cells at the concentration of 2 nM. The original format of the antibody was CD47 and SIRPα interaction blockers. The human IgG4 version of the antibody bound to hSIRPα v1, hSIRPα v2, hSIIPβ and hSIRPγ ECD by ELISA (EC50 = 0.08, 0.08, 0.08 and 6.11 nM respectively). The human IgG4 version of the antibody showed a subnanomolar EC50 for binding to CHOK1-human SIRPα v1-1B4 cells as measured by FACS (EC50 = 1.3 nM). The human IgG4 version of the antibody cross reacted with cyno SIRPα and mouse SIRPα of the C57BL/6 strain. The human IgG4 version of the antibody blocked the interactions between CD47 and SIRPα and couldn bind with B-hSIRPA mice derived primary monocytes, indicating they bind to human SIRPα IgV domain. Humanized versions of the antibody were generated and some of them maintained comparable binding capability against the recombinant proteins of human SIRPα v1 ECD, human SIRPα v2 ECD, human SIRPα v8 ECD and human SIRPβ ECD in ELISA assay, comparable species cross reactivity against human SIRPα, cyno SIRPα and C57BL/6 mouse SIRPα by FACS assay and comparable CD47 and SIRPα interaction blocking activity. The humanized versions of the antibody were confirmed to stimulate stronger or comparable macrophage phagocytosis of Jurkat cells, DLD1 cells and Raji cells. The human IgG4 and the humanized versions of the antibody did not interrupt T cell proliferation (US20210347908A1).

Gene ID

140885; 19261

Gene Name

Sirpa, SIRPA

Specificity

p84; Bit; 035; SIRPα; SIRPA; SHPS-1; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; SHP substrate 1; MyD-1 antigen; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Inhibitory receptor SHPS-1; Macrophage fusion receptor; CD172 antigen-like family member A; Tyrosine-protein phosphatase non-receptor type substrate 1; Brain Ig-like molecule with tyrosine-based activation motifs

UniProt ID

P78324, P97797

AB05165-23.0 Datasheet

Price

£430.00

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