Anti-KIT/SCFR Antibody
Catalogue Number: ABO11949-ABG
ABO11949 Image
ABO11949 Image
ABO11949 Image
ABO11949 Image
ABO11949 Image
Figure 1. Western blot analysis of C-Kit using anti-C-Kit antibody (ABO11949). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: Recombinan Human C-Kit Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # ABO11949) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C-Kit at approximately 49KD. The expected band size for C-Kit is at 49KD.
ABO11949 Image
Figure 2. Western blot analysis of C-Kit using anti-C-Kit antibody (ABO11949). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: HEPG2 Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # ABO11949) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C-Kit at approximately 109KD. The expected band size for C-Kit is at 109KD.
ABO11949 Image
Figure 3. IHC analysis of C-Kit using anti-C-Kit antibody (ABO11949).C-Kit was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-C-Kit Antibody (ABO11949) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO11949 Image
Figure 4. Flow Cytometry analysis of K562 cells using anti-C-Kit antibody (ABO11949).Overlay histogram showing K562 cells stained with ABO11949 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Kit Antibody (ABO11949,1µg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Manufacturer: | Abcepta |
| Shelf Life: | 12 months |
| Molecular Weight: | 109865 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Mast/stem cell growth factor receptor Kit, SCFR, 2.7.10.1, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit. |
| Application: | FC, ICC, IHC-P, WB, IHC-Fr, IHC |
Description
Description: Rabbit IgG polyclonal antibody for Mast/stem cell growth factor receptor Kit(KIT) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.
Additional Text
Gene ID
3815
Uniprot ID
P10721
Molecular Weight
109865
Concentration
500 ug/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
At -20°C for one year. After r°Constitution, at 4°C for one month. It°Can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Gene Name
KIT
Preparation
Lyophilized
ABO11949 Datasheet
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