Anti-CARD4 Picoband Antibody
Catalogue Number: ABO11985-ABG
ABO11985 Image
ABO11985 Image
ABO11985 Image
ABO11985 Image
ABO11985 Image
ABO11985 Image
ABO11985 Image
Figure 2. IHC analysis of CARD4 using anti-CARD4 antibody (ABO11985).CARD4 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody (ABO11985) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO11985 Image
Figure 3. IHC analysis of CARD4 using anti-CARD4 antibody (ABO11985).CARD4 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody (ABO11985) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO11985 Image
Figure 4. IHC analysis of CARD4 using anti-CARD4 antibody (ABO11985).CARD4 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody (ABO11985) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO11985 Image
Figure 5. IHC analysis of CARD4 using anti-CARD4 antibody (ABO11985).CARD4 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CARD4 Antibody (ABO11985) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
| Manufacturer: | Abcepta |
| Shelf Life: | 12 months |
| Molecular Weight: | 107691 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, NOD1, CARD4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4. |
| Application: | ICC, IHC-P, WB, IHC |
Description
Description: Rabbit IgG polyclonal antibody for Nucleotide-binding oligomerization domain-containing protein 1(NOD1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Additional Text
Gene ID
10392
Molecular Weight
107691
Concentration
500 ug/ml
Uniprot ID
Q9Y239
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
At -20°C for one year. After r°Constitution, at 4°C for one month. It°Can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Gene Name
NOD1
Preparation
Lyophilized
ABO11985 Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations