Anti-SP-D Antibody
Catalogue Number: ABO12303-ABG
ABO12303 Image
ABO12303 Image
ABO12303 Image
ABO12303 Image
ABO12303 Image
ABO12303 Image
Anti- Surfactant protein D Picoband antibody, ABO12303, Western blottingAll lanes: Anti Surfactant protein D (ABO12303) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugPredicted bind size: 38KDObserved bind size: 38KD
ABO12303 Image
Anti- Surfactant protein D Picoband antibody, ABO12303, IHC(P)IHC(P): Human Lung Cancer Tissue
ABO12303 Image
Figure 3. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO12303 Image
Figure 4. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO12303 Image
Figure 5. IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (ABO12303).Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody (ABO12303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5
| Manufacturer: | Abcepta |
| Shelf Life: | 12 months |
| Molecular Weight: | 37728 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Pulmonary surfactant-associated protein D, PSP-D, SP-D, Collectin-7, Lung surfactant protein D, SFTPD, COLEC7, PSPD, SFTP4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | A synthetic peptide corresponding to a sequence at the C-terminus of human Surfactant protein D (292-321aa RSAAENAALQQLVVAKNEAAFLSMTD SKTE), different from the related mouse and rat sequences by eight amino acids. |
| Application: | ELISA, ICC, IHC-P, WB, IHC-Fr, IHC |
Description
Description: Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein D(SFTPD) detection. Tested with WB, IHC-P, IHC-F, ICC, ELISA in Human;Mouse; Rat.
Additional Text
Uniprot ID
P35247
Gene ID
6441
Molecular Weight
37728
Concentration
500 ug/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
At -20°C for one year. After r°Constitution, at 4°C for one month. It°Can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Gene Name
SFTPD
Preparation
Lyophilized
ABO12303 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations