Anti-FASN Picoband Antibody
Catalogue Number: ABO12551-ABG
ABO12551 Image
ABO12551 Image
ABO12551 Image
ABO12551 Image
ABO12551 Image
Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody (Catalog # ABO12551) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
ABO12551 Image
FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody (Catalog # ABO12551) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA2010).
ABO12551 Image
Figure 3. IHC analysis of FASN using anti-FASN antibody (ABO12551).FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody (ABO12551) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ABO12551 Image
Figure 4. IHC analysis of FASN using anti-FASN antibody (ABO12551).FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-FASN Antibody (ABO12551) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
| Manufacturer: | Abcepta |
| Shelf Life: | 12 months |
| Molecular Weight: | 273427 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively. |
| Application: | ICC, IHC-P, WB, IHC |
Description
Description: Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Additional Text
Gene ID
2194
Uniprot ID
P49327
Molecular Weight
273427
Concentration
500 ug/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Specificity
Fatty acid synthase, 2.3.1.85, [Acyl-carrier-protein] S-acetyltransferase, 2.3.1.38, [Acyl-carrier-protein] S-malonyltransferase, 2.3.1.39, 3-oxoacyl-[acyl-carrier-protein] synthase, 2.3.1.41, 3-oxoacyl-[acyl-carrier-protein] reductase, 1.1.1.100, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 4.2.1.59, Enoyl-[acyl-carrier-protein] reductase, 1.3.1.39, Oleoyl-[acyl-carrier-protein] hydrolase, 3.1.2.14, FASN, FAS
Storage Note
At -20°C for one year. After r°Constitution, at 4°C for one month. It°Can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Gene Name
FASN
Preparation
Lyophilized
ABO12551 Datasheet
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