Anti-LFA3 Antibody
Catalogue Number: ABO12782-ABG
ABO12782 Image
ABO12782 Image
ABO12782 Image
ABO12782 Image
Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody (Catalog # ABO12782) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
ABO12782 Image
LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody (Catalog # ABO12782) at 1 µg/mL. The immunohistochemical section was developed using SABC method .
ABO12782 Image
Figure 3. Flow Cytometry analysis of Hela cells using anti-LFA3 antibody (ABO12782).Overlay histogram showing Hela cells stained with ABO12782 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (ABO12782,1µg/1x106 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Manufacturer: | Abcepta |
| Shelf Life: | 12 months |
| Molecular Weight: | 28147 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD58, CD58, LFA3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | E. coli-derived human LFA3 recombinant protein (Position: F29-R215). |
| Application: | FC, ICC, IHC-P, WB, IHC-Fr, IHC |
Description
Description: Rabbit IgG polyclonal antibody for Lymphocyte function-associated antigen 3(CD58) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.
Additional Text
Uniprot ID
P19256
Gene ID
965
Molecular Weight
28147
Concentration
500 ug/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
At -20°C for one year. After r°Constitution, at 4°C for one month. It°Can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Gene Name
CD58
Preparation
Lyophilized
ABO12782 Datasheet
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