Anti-BMP4 Picoband Antibody
Catalogue Number: ABO12824-ABG
ABO12824 Image
ABO12824 Image
Figure 1. Western blot analysis of BMP4 using anti-BMP4 antibody (ABO12824). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human BMP4 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody (Catalog # ABO12824) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BMP4 at approximately 22KD. The expected band size for BMP4 is at 13KD.
| Manufacturer: | Abcepta |
| Shelf Life: | 12 months |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | |
| Immunogen: | E. coli-derived human BMP4 recombinant protein (Position: S293-R408). |
| Application: | ELISA, WB |
Description
Description: Rabbit IgG polyclonal antibody for BMP4 detection. Tested with WB, ELISA(Cap) in Human;Mouse;Rat.
Additional Text
Gene ID
652
Gene Name
BMP4
Concentration
500 ug/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
At -20°C for one year. After r°Constitution, at 4°C for one month. It°Can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Uniprot ID
P12644
Preparation
Lyophilized
ABO12824 Datasheet
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