Functional APRIL (mouse) Antibody, mAb (recombinant) (blocking) (Biotin)
Catalogue Number: ADP0003-ABG
ADP0003 Image
ADP0003 Image
Binding of APRIL (mouse) to BCMA is inhibited by anti-APRIL (mouse), mAb (rec.) (blocking) (APRY-1-1). BCMA:Fc was coated on an ELISA plate at 1ug /ml. anti-APRIL (mouse) mAb (rec.) (blocking) (APRY-1-1) or an unrelated mAb (recombinant) (Negative control) were added (starting at 50ug/ml with a twofold serial dilution) together with 0.1ug /ml of MegaAPRIL (mouse). After incubation for 1 h at RT, the MegaAPRIL (mouse) binding was detected using an anti-FLAG antibody (HRP). The percentage of binding is shown.
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | A Proliferation Inducing Ligand; TNFSF13; CD256; TALL-2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | |
| Clone: | Apry-1-1 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, IP |
Description
Description: APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Additional Text
Concentration
1 mg/ml
Gene Name
TNFSF13
Uniprot ID
Q9D777
Gene ID
69583
Antibody Clonality
Monoclonal
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