Functional Angiopoietin-2 (human) Antibody, mAb (recombinant) (blocking)
Catalogue Number: ADP0004-ABG
ADP0004 Image
ADP0004 Image
Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4). Methods:Tie-2 (human):Fc was coated on an ELISA plate at 1 µg/ml. anti-Angiopoietin-2 (human), mAb (rec.) (blocking), (Angy-1-4) or an unrelated mAb (recombinant) (Control) were added (starting at 40 µg/ml with a twofold serial dilution) together with 200ng/ µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Ang-2; Ang2; Angpt2; Agpt2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | |
| Clone: | Angy-1-4 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA |
Description
Description: Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. anti-Angiopoietin-2 (human), mAb (rec.) (blocking) (Angy-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Additional Text
Uniprot ID
O15123
Gene Name
ANGPT2
Gene ID
285
Concentration
1 mg/ml
Antibody Clonality
Monoclonal
ADP0004 Datasheet
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