D-Alanine aminotransferase (EC 2.6.1.21), Bacillus subtilis

Catalogue Number: AE00141-NZY

Manufacturer:NZYTech
Type:Transferases
Shipping Condition:RT
Storage Condition:2-8°C
Unit(s): 2500 U

Description

Description: D-Alanine aminotransferase (EC 2.6.1.21) is purified from a recombinant E. coli strain. Alanine aminotransferase, formerly known as L-glutamic-pyruvic transaminase, is a pyridoxal phosphate-dependent enzyme present in serum and in various bodily tissues, mainly in the liver. This enzyme catalyses the transfer of an amino group from alanine to α-ketoglutarate, thus forming pyruvate and glutamate. Alanine aminotransferase is commonly used as a way of screening for liver problems. In fact, its levels significantly elevated often suggest the existence of viral hepatitis, liver damage or bile duct problems. Following damage to these cells, the enzyme is released into the blood where the level can be measured. The enzyme is provided in 3.2 M ammonium sulphate. Substrate Specificity: Under the reaction conditions specified the enzyme does not present any other detectable enzymatic activities. Activity: 1440 U/ml Temperature and pH optimum/stability: The optimum pH and temperature are 7.5 and 25 °C, respectively.

Additional Text

Purification

Purified

Short Description

D-Alanine aminotransferase (EC 2.6.1.21) is purified from a recombinant E. coli strain. This enzyme catalyses the transfer of an amino group from alanine to α-ketoglutarate, thus forming pyruvate and glutamate.