Goat Anti-Karyopherin (importin) beta 1 Antibody
Catalogue Number: AF1581A-ABG
AF1581a Image
AF1581a Image
AF1581a Image
AF1581a Image
AF1581a Image
AF1581a Image
AF1581a (0.03 µg/ml) staining of Daudi cell lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AF1581a Image
EB07018 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AF1581a Image
EB07018 (0.03µg/ml) staining of A431 (A), HEK293 (B), Jurkat (C), (0.01ug/ml) Daudi (D), HeLa (E) and (0.1ug/ml) Kelly (F) and KNRK (G) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
AF1581a Image
EB07018 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear membrane, nuclear and cytoplasmic staining
AF1581a Image
EB07018 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
| Manufacturer: | Abcepta |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97, KPNB1, NTF97 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | FC, IF, WB, IHC, pELISA |
Description
Description: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
Additional Text
Gene Name
KPNB1
Gene ID
3837
Accession Number
NP_002256
Uniprot ID
Q14974
Antigen Type
Synthetic Peptide
Antibody Clonality
Polyclonal
Short Description
Peptide-affinity purified goat antibody
Concentration
0.5 ug/ml
AF1581a Datasheet
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