CD3 zeta Antibody
Catalogue Number: AF5405-AFF
| Manufacturer: | Affinity Biosciences Ltd |
| Shelf Life: | 12 months |
| Preservative: | 0.02% sodium azide |
| Molecular Weight: | 19 kDa |
| Ph: | 7.4 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CD247 CD3Z T3Z TCRZ |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 200 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A synthesized peptide derived from human CD3 zeta, corresponding to a region within C-terminal amino acids. |
| Application: | ICC, IF, WB, pELISA |
Description
Description: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Additional Text
Concentration
1 mg/ml
Gene ID
919
Gene Name
CD247
Molecular Weight
19 kDa
Uniprot ID
P20963
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Storage Note
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
AF5405 Datasheet
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