L-Glutamine/Ammonia, UV method
Catalogue Number: AK00111-NZY
| Manufacturer: | NZYTech |
| Type: | Detection Kit |
| Shipping Condition: | RT |
| Storage Condition: | 2-8°C |
| Unit(s): | 50 tests |
| Application: | Cell culture |
Description
Description: Enzymatic method for the determination of L-glutamine and ammonia. Based on the spectrophotometric measurement of NADP+ formed through the combined action of glutaminase (GLT) and glutamate dehydrogenase (GlDH). This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products. L-Glutamine is an essential nutrient of cell culture media. However, the incorporation of this labile amino acid in growth media leads to its spontaneous break down to L-glutamate and free ammonium ions. The released ammonium ions are very toxic to cells. To overcome these issues, either pre-formulated growth media are used strictly within their recommended shelf-lives or L-glutamine is added just before use. In either case, monitoring of L-glutamine and ammonia is frequently performed both prior to, and during culturing. Related analytical kits are also available from Nzytech for ammonia (AK00091) and urea/ammonia (AK00101).
Additional Text
Detection Method
Enzymatic
Short Description
This rapid and simple specific enzymatic method is used for the simultaneous determination of L-glutamine and ammonia (ammonium ions) in cell culture media and foodstuffs such as powdered dietary supplements, bakery products and fruit and vegetable products
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