RAD23B / HR23B Antibody (aa163-176)

Catalogue Number: ALS11350-ABG

Manufacturer:	Abcepta
Molecular Weight:	43171
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	UV excision repair protein RAD23 homolog B, HR23B, hHR23B, XP-C repair-complementing complex 58 kDa protein, p58, RAD23B
Shipping Condition:	Blue Ice
Unit(s):	50 ug
Host name:	Goat
Clone:
Isotype:
Immunogen:
Application:	ELISA, IF, IHC-P, WB

Overview
Datasheets

Description

Description: Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

Additional Text

Concentration

0.5 mg/ml

Gene Name

RAD23B

Uniprot ID

P54727

Gene ID

5887

Antigen Type

Synthetic Peptide

Molecular Weight

43171

Storage Note

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Short Description

Goat Polyclonal Antibody

Purification

Affinity Purified

Antibody Clonality

Polyclonal

ALS11350 Datasheet

Price

£494.00

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