IRAK1 / IRAK Antibody (clone 3A9)
Catalogue Number: ALS13346-ABG
| Manufacturer: | Abcepta |
| Molecular Weight: | 76537 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Interleukin-1 receptor-associated kinase 1, IRAK-1, 2.7.11.1, IRAK1, IRAK |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 3A9 |
| Isotype: | |
| Immunogen: | |
| Application: | ELISA, IF, IHC-P, IP, WB, IHC, RNAi |
Description
Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
Additional Text
Gene Name
IRAK1
Uniprot ID
P51617
Gene ID
3654
Antigen Type
Recombinant Protein
Molecular Weight
76537
Concentration
0.44 mg/ml
Storage Note
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Short Description
Mouse Monoclonal Antibody
Purification
Protein A purified
Antibody Clonality
Monoclonal
ALS13346 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations