MET/HGFR Antibody
Catalogue Number: AM1001A-ABG
AM1001a Image
AM1001a Image
AM1001a Image
AM1001a Image
AM1001a Image
Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1001a MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).
AM1001a Image
All lanes : Anti-MET/HGFR Antibody at dilution Lane 1: HeLa whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: COS-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 156 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM1001a Image
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MET/HGFR Antibody (Cat.#AM1001a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AM1001a Image
Immunohistochemical analysis of paraffin-embedded H.liver section using MET/HGFR Antibody(Cat#AM1001a). AM1001a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Mouse |
| Clone: | 4AT44 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | IF, IHC-P, WB |
Description
Description: The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
Additional Text
Gene ID
4233
Gene Name
MET
Uniprot ID
P08581
Molecular Weight
155541
Antibody Clonality
Monoclonal
Accession Number
NP_000236.2,,NP_001120972.1
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM1001a Datasheet
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