GAPDH Antibody
Catalogue Number: AM1020B-ABG
AM1020b Image
AM1020b Image
AM1020b Image
AM1020b Image
AM1020b Image
AM1020b Image
AM1020b Image
AM1020b Image
Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
AM1020b Image
All lanes : Anti-GAPDH Antibody at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM1020b Image
All lanes : Anti-GAPDH Antibody at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM1020b Image
Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35µg/lane). GAPDH(arrow) was detected using the purified Mab.
AM1020b Image
Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
AM1020b Image
Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
AM1020b Image
All lanes: Anti-GAPDH Antibody at 1:1000 dilution + C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated (ASP1613) at 1/15000 dilution. Observed band size: 36KDa Blocking/Dilution buffer: 5% NFDM/TBST.
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Mouse |
| Clone: | 1A10A10 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | IF, IHC-P, WB |
Description
Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Additional Text
Gene Name
GAPDH
Gene ID
2597
Molecular Weight
36053
Uniprot ID
P04406
Antibody Clonality
Monoclonal
Accession Number
NP_001243728.1,,NP_002037.2
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM1020b Datasheet
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