Beta-actin Antibody
Catalogue Number: AM1021B-ABG
AM1021B Image
AM1021B Image
AM1021B Image
AM1021B Image
AM1021B Image
AM1021B Image
All lanes : Anti-Beta-actin Antibody at 1:4000-1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM1021B Image
Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody(Cat. #AM1021b). AM1021b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20µg per lane.
AM1021B Image
Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat. #AM1021b). AM1021b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35µg per lane.
AM1021B Image
Immunohistochemical analysis of paraffin-embedded Human Ovarian cancer section using Pink1(Cat#am1021b). am1021b was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
AM1021B Image
Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#AM1021b). AM1021b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Mouse |
| Clone: | 8H10D10 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | IHC-P, WB, IHC |
Description
Description: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Additional Text
Gene Name
ACTB
Gene ID
60
Molecular Weight
41737
Accession Number
NP_001092.1
Uniprot ID
P60709
Antibody Clonality
Monoclonal
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM1021B Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations