SUMO1 Antibody
Catalogue Number: AM1200A-ABG
AM1200a Image
AM1200a Image
AM1200a Image
AM1200a Image
AM1200a Image
AM1200a Image
AM1200a Image
AM1200a Image
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling SUMO1 with AM1200a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
AM1200a Image
The SUMO1 monoclonal antibody (Cat. #AM1200a) is used in Western blot to detect SUMO1 in HL60 cell lysate.
AM1200a Image
All lanes : Anti-SUMO1 Antibody at 1:4000 dilution Lane 1: HL-60 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM1200a Image
AM1200a staining SUMO1 in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AM1200a Image
AM1200a staining SUMO1 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AM1200a Image
Overlay histogram showing Jurkat cells stained with AM1200a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM1200a, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was mouse IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
AM1200a Image
Immunohistochemical analysis of paraffin-embedded Human Lung adenocarcinoma section using Pink1(Cat#am1200a). am1200a was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Small ubiquitin-related modifier 1, SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1, SUMO1, SMT3C, SMT3H3, UBL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Mouse |
| Clone: | 66AT1273.94 |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | FC, IF, IHC-P, WB, IHC |
Description
Description: This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
Additional Text
Gene Name
SUMO1
Uniprot ID
P63165
Gene ID
7341
Molecular Weight
11557
Antibody Clonality
Monoclonal
Accession Number
NP_001005781.1,,NP_001005782.1,,NP_003343.1
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM1200a Datasheet
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