BRAF Antibody
Catalogue Number: AM1821A-ABG
AM1821a Image
AM1821a Image
AM1821a Image
AM1821a Image
Fluorescent image of C2C12 cell stained with BRAF Antibody(Cat#AM1821a/SG100521B).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37°C. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C.BRAF immunoreactivity is localized to Cytoplasm significantly.
AM1821a Image
All lanes : Anti-BRAF Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM1821a Image
Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35µg/lane). BRAF (arrow) was detected using the purified Mab.
| Manufacturer: | Abcepta |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Mouse |
| Clone: | 125CT13.3.5 |
| Isotype: | IgG1, kappa |
| Immunogen: | |
| Application: | IF, WB |
Description
Description: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Additional Text
Gene Name
BRAF
Uniprot ID
P15056
Gene ID
673
Antigen Type
Recombinant Protein
Molecular Weight
84437
Antibody Clonality
Monoclonal
Accession Number
NP_004324,,NP_004324.2
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM1821a Datasheet
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