RAB27B Antibody
Catalogue Number: AM8688B-ABG
AM8688B Image
AM8688B Image
AM8688B Image
All lanes : Anti-RAB27B Antibody at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: mouse stomach lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM8688B Image
Overlay histogram showing A431 cells stained with AM8688b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8688b, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was mouse IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
| Manufacturer: | Abcepta |
| Molecular Weight: | 24608 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Ras-related protein Rab-27B, C25KG, RAB27B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 1596CT245.254.49 |
| Isotype: | IgG1, kappa |
| Immunogen: | |
| Application: | FC, WB |
Description
Description: May be involved in targeting uroplakins to urothelial apical membranes.
Additional Text
Uniprot ID
O00194
Gene ID
5874
Molecular Weight
24608
Antibody Clonality
Monoclonal
Gene Name
Rab27b
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM8688B Datasheet
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