PLIN3 Antibody
Catalogue Number: AM8697B-ABG
AM8697B Image
AM8697B Image
AM8697B Image
All lanes : Anti-PLIN3 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AM8697B Image
Overlay histogram showing HUVEC cells stained with AM8697b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8697b, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was mouse IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
| Manufacturer: | Abcepta |
| Molecular Weight: | 47075 |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein, Cargo selection protein TIP47, Mannose-6-phosphate receptor-binding protein 1, Placental protein 17, PP17, PLIN3, M6PRBP1, TIP47 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul, 50 ul |
| Host name: | Mouse |
| Clone: | 1651CT490.66.85 |
| Isotype: | IgG2a, kappa |
| Immunogen: | |
| Application: | FC, WB |
Description
Description: Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
Additional Text
Uniprot ID
O60664
Gene ID
10226
Antigen Type
Recombinant Protein
Molecular Weight
47075
Antibody Clonality
Monoclonal
Gene Name
PLIN3
Short Description
Purified Mouse Monoclonal Antibody (Mab)
Preparation
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
AM8697B Datasheet
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