Phospho-Ser774 Dynamin Antibody
Catalogue Number: AN1007-ABG
AN1007 Image
AN1007 Image
Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with λ-Ptase.
| Manufacturer: | Abcepta |
| Molecular Weight: | 97295 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Dynamin-1, B-dynamin, D100, Dynamin, brain, Dnm1, Dnm |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Sheep |
| Clone: | |
| Isotype: | |
| Immunogen: | |
| Application: | WB |
Description
Description: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010)
Additional Text
Gene Name
DNM1
Gene ID
140694
Uniprot ID
P21575
Antibody Clonality
Polyclonal
Short Description
Affinity purified sheep polyclonal antibody
Molecular Weight
97295
Preparation
Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
AN1007 Datasheet
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