HA Tag Antibody
Catalogue Number: AP1012A-ABG
AP1012A Image
AP1012A Image
AP1012A Image
AP1012A Image
AP1012A Image
AP1012A Image
GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
AP1012A Image
ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))
AP1012A Image
Western blot analysis of Tag-HA. 2x Antibody (arrow) using rabbit polyclonal AP1012A (Cat. #AP1012A). 293T/17 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with 41kDa HA tagged vector.
AP1012A Image
Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody (Cat. #AP1012A). AP1012A was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
AP1012A Image
Western blot analysis of anti-HA tag pab(Cat. #AP1012a)in HA-tagged recombinant protein bacterial lysate.
| Manufacturer: | Abcepta |
| Type: | ChIP Validated Antibodies |
| Alias: | Glutathione S-transferase |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | RB0411 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | IF, ChIP, WB |
Description
Description: Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Additional Text
Antigen Type
Synthetic Peptide
Antibody Clonality
Polyclonal
Short Description
Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
AP1012A Datasheet
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