MART-1/Melan-A Antibody (Center)
Catalogue Number: AP11689C-ABG
AP11689c Image
AP11689c Image
AP11689c Image
AP11689c Image
MART-1/Melan-A Antibody (Center) (Cat. #AP11689c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MART-1/Melan-A antibody detected the MART-1/Melan-A protein (arrow).
AP11689c Image
Anti-MLANA Antibody (Center) at 1:2000 dilution + SK-MEL-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP11689c Image
Overlay histogram showing A2058 cells stained with AP11689c(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
| Manufacturer: | Abcepta |
| Molecular Weight: | 13157 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB19079 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, WB |
Description
Description: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein SILV/PMEL17, which is critical to the formation of stage II melanosomes.
Additional Text
Gene Name
MLANA
Uniprot ID
Q16655
Gene ID
2315
Antigen Type
Synthetic Peptide
Molecular Weight
13157
Accession Number
NP_005502.1
Antibody Clonality
Polyclonal
Short Description
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP11689c Datasheet
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