PURB Antibody (C-term)
Catalogue Number: AP12051B-ABG
AP12051B Image
AP12051B Image
AP12051B Image
AP12051B Image
Fluorescent image of A549 cell stained with PURB Antibody (C-term)(Cat#AP12051b).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PURB primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).PURB immunoreactivity is localized to Nucleus significantly.
AP12051B Image
PURB Antibody (C-term) (Cat. #AP12051b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PURB antibody detected the PURB protein (arrow).
AP12051B Image
PURB Antibody (C-term) (Cat. #AP12051b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
| Manufacturer: | Abcepta |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB32000 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, IF, WB |
Description
Description: This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
Additional Text
Antigen Type
Synthetic Peptide
Gene ID
5814
Accession Number
NP_150093.1
Uniprot ID
O35295, Q68A21, Q96QR8
Antibody Clonality
Polyclonal
Gene Name
PURB
Short Description
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight
33241
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP12051B Datasheet
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