ND4L Antibody (C-term)
Catalogue Number: AP17147B-ABG
AP17147b Image
AP17147b Image
AP17147b Image
AP17147b Image
All lanes : Anti-ND4L Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP17147b Image
Anti-ND4L Antibody (C-term) at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP17147b Image
Overlay histogram showing U-2 OS cells stained with AP17147b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17147b, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
| Manufacturer: | Abcepta |
| Molecular Weight: | 10741 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | NADH-ubiquinone oxidoreductase chain 4L, NADH dehydrogenase subunit 4L, MT-ND4L, MTND4L, NADH4L, ND4L |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB35904 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, WB |
Description
Description: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Additional Text
Gene ID
4539
Antigen Type
Synthetic Peptide
Antibody Clonality
Polyclonal
Accession Number
YP_003024034.1
Uniprot ID
P03901, Q7J3C3
Gene Name
ND4L
Short Description
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight
10741
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP17147b Datasheet
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