ADRA1D Antibody (N-term)
Catalogue Number: AP20589A-ABG
AP20589a Image
AP20589a Image
AP20589a Image
AP20589a Image
AP20589a Image
AP20589a Image
Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody (N-term) (Cat. #AP20589a). AP20589a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
AP20589a Image
Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term)(Cat#AP20589a). AP20589a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
AP20589a Image
Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term)(Cat#AP20589a). AP20589a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
AP20589a Image
Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term)(Cat#AP20589a). AP20589a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
AP20589a Image
Overlay histogram showing MCF-7 cells stained with AP20589a(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP20589a, 1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
| Manufacturer: | Abcepta |
| Molecular Weight: | 60463 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB46798 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, IHC-P, WB |
Description
Description: This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Additional Text
Gene ID
146
Gene Name
ADRA1D
Uniprot ID
P25100
Antigen Type
Synthetic Peptide
Molecular Weight
60463
Antibody Clonality
Polyclonal
Short Description
Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP20589a Datasheet
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