p53 (S376) antibody
Catalogue Number: AP22298A-ABG
AP22298a Image
AP22298a Image
AP22298a Image
Anti- p53 (S376) Antibody at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP22298a Image
All lanes : Anti-p53(S376) Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
| Manufacturer: | Abcepta |
| Molecular Weight: | 43653 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul, 50 ul |
| Host name: | Rabbit |
| Clone: | RB57840 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | WB |
Description
Description: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Additional Text
Gene Name
TP53
Gene ID
7157
Uniprot ID
P04637
Antigen Type
Synthetic Peptide
Molecular Weight
43653
Antibody Clonality
Polyclonal
Short Description
Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP22298a Datasheet
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