Me2-H4(K20) Antibody
Catalogue Number: AP3674A-ABG
AP3674a Image
AP3674a Image
AP3674a Image
AP3674a Image
AP3674a Image
Western blot analysis of lysate from K562 cell line, using H4 Antibody (K20)(Cat. #AP3674a). AP3674a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
AP3674a Image
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP3674a Image
H4 Antibody ( K20 [Me2] ) (Cat. #AP3674a) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP3674a Image
Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab (Cat. AP3674a) on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
| Manufacturer: | Abcepta |
| Molecular Weight: | 11367 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Histone H4, HIST1H4A, H4/A, H4FA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB17115 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, IHC-P, WB, DB |
Description
Description: Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.
Additional Text
Uniprot ID
P62805
Antigen Type
Synthetic Peptide
Molecular Weight
11367
Gene ID
121504; 554313; 8294; 8359; 8360; 8361; 8362; 8363; 8364; 8365; 8366; 8367; 8368; 8370
Antibody Clonality
Polyclonal
Accession Number
NP_001029249.1,,NP_003486.1,,NP_003529.1,,NP_003530.1,,NP_003531.1,,NP_003532.1,,NP_003533.1,,NP_003534.1,,NP_003535.1,,NP_003536.1,,NP_003537.1,,NP_003539.1,,NP_068803.1,,NP_778224.1
Gene Name
H4-16, H4C1, H4C11, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9
Short Description
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP3674a Datasheet
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