S100B Antibody
Catalogue Number: AP5074D-ABG
AP5074D Image
AP5074D Image
AP5074D Image
AP5074D Image
AP5074D Image
AP5074D Image
AP5074D Image
AP5074D Image
Anti-S100B Antibody at 1:2000 dilution + Human brain whole tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP5074D Image
Western blot analysis of lysates from A431 cell line, mouse brain, rat brain tissue (from left to right), using S100B Antibody(Cat. #AP5074d). AP5074d was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
AP5074D Image
All lanes : Anti-S100B Antibody at 1:2000 dilution Lane 1: Human brain lysate Lane 2: C2C12 whole cell lysate Lane 3: Rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP5074D Image
Immunohistochemical analysis of paraffin-embedded Human breast tissue using AP5074D performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
AP5074D Image
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue using AP5074D performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
AP5074D Image
S100B Antibody (Cat. #AP5074d) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AP5074D Image
Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1(Cat#AP5074D). AP5074D was diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
| Manufacturer: | Abcepta |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB22934 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | FC, IHC-P, WB, IHC |
Description
Description: S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Additional Text
Gene Name
S100B
Accession Number
NP_006263.1
Gene ID
6285
Antigen Type
Recombinant Protein
Molecular Weight
10713
Antibody Clonality
Polyclonal
Uniprot ID
P04271, Q6YNR6
Short Description
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP5074D Datasheet
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