CUX1 Antibody (C-term)
Catalogue Number: AP5895B-ABG
AP5895B Image
AP5895B Image
AP5895B Image
AP5895B Image
AP5895B Image
AP5895B Image
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling CUX1 with AP5895b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
AP5895B Image
All lanes : Anti-CUX1 Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 164 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP5895B Image
All lanes : Anti-CUX1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 164 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP5895B Image
AP5895b staining CUX1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AP5895B Image
AP5895b staining CUX1 in human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
| Manufacturer: | Abcepta |
| Molecular Weight: | 164187 |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Homeobox protein cut-like 1, CCAAT displacement protein, CDP, Homeobox protein cux-1, CUX1, CUTL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 400 ul, 80 ul |
| Host name: | Rabbit |
| Clone: | RB23382 |
| Isotype: | IgG |
| Immunogen: | |
| Application: | IF, IHC-P, WB |
Additional Text
Gene Name
CUX1
Uniprot ID
P39880
Gene ID
1523
Antigen Type
Synthetic Peptide
Molecular Weight
164187
Antibody Clonality
Polyclonal
Accession Number
NP_001189472.1,,NP_001189473.1,,NP_001189474.1,,NP_852477.1,,NP_853530.2
Short Description
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Preparation
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
AP5895B Datasheet
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