HighFidelity AZDye647 PCR Labeling Kit, Preparation of AZDye647-labeled DNA probes by PCR
Catalogue Number: APP-101-AZ647-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | PCR Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 reactions |
| Application: | PCR |
Description
Description: HighFidelity AZDye647 PCR Labeling Kit is designed to produce randomly AZDye647-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4kbp is feasible. dUTP-XX-AZDye647 is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50 % dUTP-XX-AZDye647 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-AZDye647/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-XX-AZDye647 substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-XX-AZDye647).
Additional Text
Smile String
P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC[N+]1=C(C(C)(c2cc(ccc12)S(=O)(=O)O)CCCCS(=O)(=O)[O-])/C=C/C=C/C=C1/N(c2ccc(cc2C1(C)C)S(=O)(=O)O)CCCS(=O)(=O)O
Storage Note
store at -20 °C|12 months|avoid freeze/thaw cycles, store dark
APP-101-AZ647-L Datasheet
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