HighFidelity Cy3 PCR Labeling Kit, Preparation of Cy3-labeled DNA probes by PCR
Catalogue Number: APP-101-CY3-S-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Type: | Nucleic Acid Labeling Kits |
| Shipping Condition: | Blue Ice |
| Unit(s): | 10 reactions |
| Application: | PCR |
Description
Description: HighFidelity Cy3 PCR Labeling Kit is designed to produce randomly Cy3-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4kbp is feasible. dUTP-XX-Cy3 is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50 % dUTP-XX-Cy3 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-Cy3/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-XX-Cy3 substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-XX-Cy3).
Additional Text
Smile String
C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=CC=CC1=[N+](c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC
Storage Note
store at -20 °C|12 months|avoid freeze/thaw cycles, store dark
APP-101-CY3-S Datasheet
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