Alpha-tubulin Antibody

Catalogue Number: ASC10875-ABG

Manufacturer:Abcepta
Shelf Life:12 months
Physical state:Liquid
Type:Polyclonal Primary Antibody - Unconjugated
Shipping Condition:Blue Ice
Unit(s): 0.1 mg
Host name: Chicken
Clone:
Isotype: IgY
Immunogen:
Application: ELISA, WB

Description

Description: Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.

Additional Text

Uniprot ID

Q13748

Accession Number

NP_005992

Application Notes

Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 - 1 µg/mL.

Purification

Affinity Purified

Gene ID

113457; 7278

Gene Name

TUBA3C, TUBA3D

Antibody Clonality

Polyclonal

Storage Note

Alpha-tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.