HSP70 Antibody
Catalogue Number: ASM10022-ABG
ASM10022 Image
ASM10022 Image
ASM10022 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1.86 (ASM10022). Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (ASM10022) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.
ASM10022 Image
Western Blot analysis of Human HEK293 cell lysate showing detection of ~70 kDa Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 1.86 (ASM10022). Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (ASM10022) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Bovine HSP70 |
| Application: | WB |
Additional Text
Gene Name
HSPA1A
Purification
Protein G purified
Uniprot ID
Q27975
Gene ID
282254
Accession Number
NP_776975.1
Storage Note
-20C
ASM10022 Datasheet
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