HSF1 Antibody

Catalogue Number: ASM10028-BI-ABG

ASM10028-BI Image

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite.

ASM10028-BI Image

Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:1000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

ASM10028-BI Image

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Heat Shocked mitotic HeLa cells. Species: Human. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:1000. Courtesy of: Morimoto Lab, Northwestern University, USA.

ASM10028-BI Image

Western Blot analysis of Human Heat Shocked HeLa cell lysates showing detection of ~85-95 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Lane 1: MW ladder. Lane 2: HeLa cell lysates (Control). Lane 3: Heat-shocked HeLa cell lysates. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~85-95 kDa.

ASM10028-BI Image

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite.

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Manufacturer:	Abcepta
Type:	Primary Antibody
Shipping Condition:	Blue Ice
Unit(s):	each
Host name:
Clone:
Isotype:	IgG1
Immunogen:	Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
Application:	WB, IHC