FIH Antibody

Catalogue Number: ASM10125-A565-ABG

ASM10125-A565 Image

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite.

ASM10125-A565 Image

Immunohistochemistry analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All positive.

ASM10125-A565 Image

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FIH Monoclonal Antibody, Clone fih162C (ASM10125). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-FIH Antibody. (C) Composite.

ASM10125-A565 Image

Western Blot analysis of Human Cell lysates showing detection of FIH protein using Mouse Anti-FIH Monoclonal Antibody, Clone fih162c (ASM10125). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FIH Monoclonal Antibody (ASM10125) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

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Manufacturer:	Abcepta
Type:	Primary Antibody
Shipping Condition:	Blue Ice
Unit(s):	each
Host name:
Clone:
Isotype:	IgG1
Immunogen:	Full length human FIH expressed in E.coli BL21 (DE3) cells
Application:	WB, IHC