HIF2 alpha Antibody
Catalogue Number: ASM10130-A565-ABG
ASM10130-A565 Image
ASM10130-A565 Image
ASM10130-A565 Image
ASM10130-A565 Image
ASM10130-A565 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite.
ASM10130-A565 Image
Western Blot analysis of Human HeLa cell lysates showing detection of HIF2 alpha protein using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Cells CoCl treated.
ASM10130-A565 Image
Immunohistochemistry analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Basal cell staining in the epidermis, dermis positive and muscle positive. .
ASM10130-A565 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HIF2 alpha Monoclonal Antibody, Clone EP190b (ASM10130). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-HIF2 alpha Monoclonal Antibody (ASM10130) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Localizes to the nucleus upon hypoxia. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HIF2 alpha Antibody. (C) Composite.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein |
| Application: | WB, IHC |
Additional Text
Gene Name
EPAS1
Gene ID
2034
Purification
Protein G purified
Uniprot ID
Q99814
Accession Number
NP_001421.4
Storage Note
-20C
ASM10130-A565 Datasheet
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