HSP47 Antibody
Catalogue Number: ASM10150-A633-ABG
ASM10150-A633 Image
ASM10150-A633 Image
ASM10150-A633 Image
ASM10150-A633 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 (ASM10150). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody (ASM10150) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite.
ASM10150-A633 Image
Western Blot analysis of Human Epithelial cell (A431) lysates showing detection of ~47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 (ASM10150). Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody (ASM10150) at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~47 kDa.
ASM10150-A633 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 (ASM10150). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody (ASM10150) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1, kappa |
| Immunogen: | Human HSP47, full length |
| Application: | WB |
Additional Text
Accession Number
NP_001193943
Purification
Protein G purified
Uniprot ID
P50454.2
Storage Note
-20C
ASM10150-A633 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations