ERp57 Antibody
Catalogue Number: ASM10174-A655-ABG
ASM10174-A655 Image
ASM10174-A655 Image
ASM10174-A655 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 (ASM10174). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody (ASM10174) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.
ASM10174-A655 Image
Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9 (ASM10174). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (ASM10174) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2b |
| Immunogen: | Human recombinant ERp57 (Grp58). |
| Application: | WB |
Additional Text
Gene Name
PDIA3
Uniprot ID
P30101
Gene ID
2923
Accession Number
NP_005304.3
Purification
Protein G purified
Storage Note
-20C
ASM10174-A655 Datasheet
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