HCN1 Antibody

Catalogue Number: ASM10182-A633-ABG

ASM10182-A633 Image

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.

ASM10182-A633 Image

Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

ASM10182-A633 Image

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

ASM10182-A633 Image

Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70-28 (ASM10182). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (ASM10182) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Neurons.

ASM10182-A633 Image

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Manufacturer:	Abcepta
Type:	Primary Antibody
Shipping Condition:	Blue Ice
Unit(s):	each
Host name:
Clone:
Isotype:	IgG1
Immunogen:	Fusion protein amino acids 778-910 (C terminus) of rat HCN1
Application:	WB, IHC