Copper Transporting ATPase 1 Antibody
Catalogue Number: ASM10232-A680-ABG
ASM10232-A680 Image
ASM10232-A680 Image
ASM10232-A680 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (ASM10232). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (ASM10232) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Copper Transporting ATPase 1 Antibody (D) Composite.
ASM10232-A680 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~180 kDa Copper Transporting ATPase 1 protein using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (ASM10232). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (ASM10232) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~180 kDa. Other Band(s): 250kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1 |
| Application: | WB |
Additional Text
Gene ID
538
Gene Name
ATP7A
Uniprot ID
Q04656
Accession Number
NP_000043.3
Purification
Protein G purified
Storage Note
-20C
ASM10232-A680 Datasheet
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