Copper Transporting ATPase 2 Antibody
Catalogue Number: ASM10233-A700-ABG
ASM10233-A700 Image
ASM10233-A700 Image
ASM10233-A700 Image
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (ASM10233). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (ASM10233) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Copper Transporting ATPase 2 Antibody (D) Composite.
ASM10233-A700 Image
Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (ASM10233). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (ASM10233) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.
| Manufacturer: | Abcepta |
| Type: | Primary Antibody |
| Shipping Condition: | Blue Ice |
| Unit(s): | each |
| Host name: | |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2 |
| Application: | WB |
Additional Text
Gene Name
ATP7B
Gene ID
540
Accession Number
NP_000044.2
Purification
Protein G purified
Uniprot ID
B7ZLR4
Storage Note
-20C
ASM10233-A700 Datasheet
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